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Figure 5 | Journal of Biomedical Science

Figure 5

From: Human parvovirus B19 nonstructural protein NS1 enhanced the expression of cleavage of 70 kDa U1-snRNP autoantigen

Figure 5

Recognition of U1-snRNP in COS-7 cells by serum from patients with B19 infection. (A) Presence of 70 kDa U1-snRNP and its cleaved 40 kDa-form in COS-7 cells transfected with pEGFP, pEGFP-NS1 and pEGFP-NS1K334E were detected with serum from B19 infected patients. Lanes 1 to 3 indicate serum from 3 different patients with B 19 infection. Actin is used as control (lane 4). Lanes 5 to 6 indicates representative results of serum samples that were randomly selected from the 10 healthy individuals. (B) Ratio of 40 kDa U1-snRNP to actin. Three independent tests were performed. * and # indicate significant difference as compared to E or N, respectively. E, N and K indicate EGFP, EGFP-NS1 and EGFP-NS1K334E, respectively.

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Journal of Biomedical Science

ISSN: 1423-0127