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Fig. 2 | Journal of Biomedical Science

Fig. 2

From: Excess glucose alone depress young mesenchymal stromal/stem cell osteogenesis and mitochondria activity within hours/days via NAD+/SIRT1 axis

Fig. 2

High glucose (HG) alone can commit human and murine MSCs towards adipogenesis while inhibiting osteogenesis. A and B Representative data and pooled data of lipid droplet accumulation as assessed in human BMMSCs (top panel), human placental MSCs (PMSCs; middle panel), or murine BMMSCs (bottom panel) by staining with Oil Red (OR). Scale bar, 50 μm. C and D Representative and pooled data of extracellular mineralization as assessed in human BMMSCs (top panel), human PMSCs (middle panel), or murine BMMSCs (bottom panel) by staining with Alizarin Red (AR). Scale bar, 50 μm. E and F qPCR analyses of functionally adipogenic and osteogenic genes, adiponectin (ADIPOQ) and bone sialoprotein (IBSP), in human BMMSCs (E) and PMSCs (F), treated with LG or HG for 3 days. G and H qPCR analyses of Adipoq and Ibsp in murine BMMSCs (G) and mouse C3H10T1/2 mesenchymal progenitor/stem cells (C3H; H), treated with LG or HG for 3 days. N = 3 for each group in all figures. Data are shown as mean ± SD. *, p < 0.05; **, p < 0.01; ***, p < 0.001

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Journal of Biomedical Science

ISSN: 1423-0127