Fig. 1
Targeting REV1 enhances lung cancer radiosensitivity in vivo but not in vitro. A Control or stable knockdown REV1 A549 lung cancer cells were selected to construct a subcutaneous transplantation tumor model in nude mice, which were then randomly divided into four groups. A total of 10 Gy x 1 F radiation treatment was given to the radiotherapy group after the tumor reached 100 mm3, and the tumor volume was measured every three days. Data are shown as the mean tumor volume ± SEM. **P < 0.01, ***P < 0.001 (n = 8 or 7 mice/group). B Weight of transplanted tumors in mice of each group in panel A. **P < 0.01, ***P < 0.001 (n = 8 or 7/group). C A549 cells were transfected with control or siREV1 for 48 h, digested, and counted. Cells were irradiated with 0, 2, 4, 6, and 8 Gy X-rays after wall attachment, and the number of cell groupings with more than 50 cells were considered colonies and was recorded after 14 days of culture to obtain the cell survival curve. D A549 lung cancer cells were used to generate a subcutaneous transplantation tumor model in nude mice and mice were randomly divided into four groups. JH-RE-06 was used at a final concentration of 1.6 mg/kg, and the drug was administered by intraperitoneal injection twice a week beginning once the tumor volume reached 100 mm3. The experimental group requiring radiotherapy was subjected to 10 Gy of radiotherapy 1 day after drug administration, and tumor volume was measured every three days. Data are shown as the mean tumor volume ± SEM. **P < 0.01 (n = 8 or 7 mice/group). The tumor doubling time (TDT) for Vehicle, JH-RE-06, Vehicle + IR, and JH-RE-06 + IR was 2.82 ± 0.58 days, 4.06 ± 1.13 days, 4.01 ± 0.30 days, and 5.98 ± 2.00 days, respectively. The enhancement factor (EF) of JH-RE-06 was 1.61. E Weight of transplanted tumors in each group of mice in the D graph. ***P < 0.001 (n = 8 or 7/group). F After treatment of A549 cells with different concentrations of JH-RE-06 for 24 h, digestions were counted on spread plates, and cells were irradiated with corresponding doses of X-ray after wall attachment. After 14 days, the number of cell spheres with more than 50 cells was recorded, and cell survival curves were depicted