Fig. 2

Cholestatic MC38-liver metastasis has an immunosuppressive microenvironment associated with neutrophil accumulation and T cell exclusion. A GO biological process analyses of DEGs of the LM of Sham/BDL MC38-bearing BT model mice. B CIBERSORT analysis of TME cells in the LM of Sham/BDL mice. The thick lines represent the median values. The bottom and top of the boxes represent the 25th and 75th percentiles (interquartile range), respectively. The whiskers encompass 1.5 times the interquartile range. C-E Flow cytometric analysis of neutrophils (C), total CD4⁺ T cells (D), total CD8⁺ T cells and PD-1⁺CD8⁺ T cells (E) of MC38-bearing BT model mice. F, Heat map of lymphocyte activation related genes between Sham and BDL mice. G-H GSEA result of the positive regulation of leukocyte differentiation pathway (G) and related mRNA expression in LM tissues (H). I, J GSEA result of the leukocyte activation pathway and T cell receptor signaling pathway (I) and related mRNA expression in LM tissues (J). Data represent mean ± SEM of 3 biologically independent experiments (Two-tailed t test). ns, no significance; Sham, sham surgery; BDL, bile duct ligation surgery