Fig. 3
Ferroptosis upregulation in ischemic mice. A Proteomic analysis reported several proteins altered in FAL-ischemic mice (IC, untreated and AT or CB treated mice) compared to sham controls. A graphical representation of the changes seen for some of them is shown (representing the Log2 of the LFQ intensities registered by MS analysis). B A western-blot analysis was done to evaluated the expression changes for the protein GPX4 in the different groups (SH, IC, AT, CB, n:6 per condition). The image registered after staining the membrane with ponceau was used for normalization as loading control (LC). C Graphical representation of the expression changes detected by western-blot for GPX4, normalized versus shams intensities. D Graphical representation of some proteins identified associated to Ferroptosis. E The levels of Malondialdehyde (MDA) were also measured with a specific colorimetric assay, representing the concentration of MDA (mmol/mgprot) per group (n:8/group). Although no statistically significant differences were seen, the tendency indicated an increase of MDA in ischemic mice compared to shams. F Changes detected in ischemic mice representative of an upregulation of ferroptosis. Proteins included here: Transferrin receptor (TFRC); Thioredoxin reductase 1 (TXNRD1); Acyl-CoA synthetase long-chain family member 4 (ACSL4); Glutathione peroxidase 4 (GPX4); NAD(P)H quinone oxidoreductase 1 (NQO1)