Fig. 5
Pre- or post-synaptic deletion of CPEB2 impairs glutamatergic transmission and LTP in the TA-CA1 circuit. A The virus injection sites in the entorhinal cortex (EC, left) and CA1 (right) were marked in green dots. B-G CPEB2-cWT mice were injected with AAV8-GFP (4.2 × 109 vg) or AAV9-Cre-ires-GFP (1.62 × 1010 vg) to generate cWT control or CPEB2-cKOEC and -cKOCA1 mice, respectively. Four weeks after intracranial delivery of AAV, 3–5-month-old male mice were used for field recording. The input–output responses (B and E), paired-pulse facilitation (C and F) and 4X HFS-evoked LTP (D and G) in the TA-CA1 pathway of CPEB2-cKOEC and -cKOCA1 hippocampal slices, respectively. Numbers in parentheses (n/N) represent the number of recorded slices (n) and mice (N). Sample traces were presented in the same manner as described in Fig. 1. Data are mean ± SEM. *P < 0.05, **P < 0.01 and ***P < 0.001, two-way ANOVA with Fisher’s LSD post hoc test