Fig. 3

PLXNC1 is involved in intracellular LPS processing in vivo and ex vivo. PLXNC1^-/- mice and littermate controls were exposed to intraperitoneal E. coli injection and survival was monitored. A PLXNC1^-/- animals and littermate controls survival curves after intraperitoneal injection of viable E. coli. BMDMs were isolated from PLXNC1^-/- mice and their littermate controls and transfected with LPS to activate caspase-11. The levels of B LDH, C IL-1ß, D IL-18 and E caspase-11 were measured in cell culture supernatant through ELISA. In lysed cells protein of respective LPS transfected BMDMs, F caspase-1 (asterisk marks the full form at around 50 kDa; arrow points at cleaved caspase-1 p20), G caspase-11 (asterisk marks 43 kDa; arrow points at 38 kDa), H gasdermin D (asterisk marks full form at around 50 kDa; arrow points at cleaved gasdermin D at around 31 kDa) and I NLRP3 expression (arrow points at full size receptor at around 110 kDa) was examined through Western blot analysis. (The data are presented as the means ± SEMs; n = 5–18 mice/group; Western blots, n = 4–7 mice/group; *p < 0.05; **p < 0.01 and ***p < 0.001 as indicated)