Fig. 1
pcMSC-CM and E2-CM improved ovary function in CTX-induced POI animal model. A Experimental schedule and design. B Changes in bodyweights of mice in four experimental groups (control, POI, CM, and E2-CM groups). C Tissue morphology (a), volume (b), and weight (c) of ovaries of mice in four experimental groups (n = 10); volume (mm3) = length × width2 × π/6. D (a) HE staining results indicate folliculogenesis in ovary tissues of mice in each group. Quantitative analysis of (b) total follicle number and (c) follicle number at various folliculogenesis stages in each group (n = 5). E Serum hormone levels of E2 (a), FSH (b), and (c) AMH in each group. Results obtained through ELISA assay. F Protein levels of steroidogenesis pathway (CYP19A1 and StAR), as detected using Western-blot assay. Data are expressed as means ± SDs of at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 compared with control group. One-way and two-way ANOVA with Tukey’s test