Fig. 3

pcMSC-CM and E2-CM restore granulosa cell viability in CTX-challenged KGN cell model. A Apoptosis of KGN cells in four experimental groups: control, Mock (4-OOH-CP treatment), CM (4-OOH-CP treatment plus CM), and E2-CM (4-OOH-CP treatment plus E2-CM) (a). Results obtained through flow cytometry analysis. Results of quantitative analysis of (a) are presented in (b) (n = 3). B Levels of cleaved caspase-3 (in green), actin filaments (in red), and DAPI (in blue) (n = 4). C Ki67 levels in KGN cells (n = 4). Results obtained through confocal immunofluorescent analysis. Results of quantitative analysis of (a) are presented in (b). *p < 0.05, **p < 0.01, ***p < 0.001. One-way ANOVA with Tukey’s test