Fig. 7

ER⁺pcMSC-derived exosomes effectively restore the defects induced by 4-OOH-CP (8 μg/ml for 12 h) in the KGN cell model. The experimental groups included the control group (Control group), the groups challenged with 4-OOH-CP then treated with: MDCD201 mock medium only (Mock group), exosome-depleted conditioned medium (Exo-/CM group), exosomes only (Exo group), and conditioned medium (CM group). A The effects of the various experimental groups on the protein expression of cleaved caspase-3 were shown. Immunofluorescence staining and quantitative analysis (a, b; n = 4) were performed, along with immunoblotting (c; n = 4). B The protein expressions of Ki67 were shown. Immunofluorescence staining and quantitative analysis (a, b; n = 4) were conducted, along with immunoblotting for PCNA (c; n = 4). C The protein expression levels of REV-ERBα in various experimental groups were shown. Immunofluorescence staining and quantitative analysis (a, b; n = 4) were performed, along with immunoblotting for PCNA (c; n = 4). *p < 0.05, **p < 0.01, ***p < 0.001. One-way ANOVA with Tukey’s test