Fig. 2

In vivo pro-angiogenic effects of DPSC-CMs produced in normoxia and hypoxia. The pro-angiogenic effects of DPSC-CMs were investigated by Ultimatrix sponge assay. A, B Representative excised Ultimatrix sponges associated with DPSC-CM Normox and DPSC-CM Hypox; C, D representative H&E images of sections from Ultimatrix sponges associated with DPSC-CM Normox and DPSC-CM Hypox showing vascular-like structures (circles) and mature blood vessels (arrows) respectively. Scale bar 20 μm; E hemoglobin content, detected by Drabkin’s assay, in sponges associated with DPSC-CM Normox and DPSC-CM Hypox; F vessel count in Ultimatrix sponges associated with DPSC-CM Normox and DPSC-CM Hypox; G flow cytometry analysis for the content of stromal CD45⁻ cells, CD31⁺ endothelial cells, CD45⁺ total leukocytes, total F4/80⁺ macrophages, CD80⁺ M1 and CD206⁺ M2 macrophages in sponges associated with DPSC-CM Normox and DPSC-CM Hypox. Results are shown as mean ± SEM, t-student test, *p ≤ 0.05; **p ≤ 0.01. Experiments were performed using CMs from 3 donors