Fig. 5

In vitro pro-angiogenic effects of DPSC-CMs produced in normoxia and hypoxia, compared to selected soluble factors. The pro-angiogenic effects of DPSC-CM Normox and DPSC-CM Hypox were tested in vitro on Human Umbilical-Vein Endothelial cells (HUVECs). A Graphs showing the most up-regulated pro-angiogenic factors in DPSC-CM Normox and DPSC-CM Hypox, as revealed by secretome analysis; B detection of HUVEC cell adhesion on fibronectin, upon stimulation with DPSC-CM Normox and DPSC-CM Hypox (magnification 10×); C detection of HUVEC cell migration on fibronectin towards DPSC-CM Normox and DPSC-CM Hypox, at 50 μg/mL of total protein or as 1:4 dilution, or IL-6 (50 ng/mL), IL-8 (20 ng/mL), SDF1 (100 ng/mL), or their combination (magnification 10×); D real-time PCR for pro-angiogenic factors VEGF-A, IL-8, CXCR4, IL-6, STAT3 expression of HUVECs stimulated with DPSC-CM Normox and DPSC-CM Hypox; E tube formation assay on HUVECs stimulated with DPSC-CM Normox and DPSC-CM Hypox (magnification 10×). Results are shown as mean ± SEM, t-student test or One-Way ANOVA, *p ≤ 0.05; **p ≤ 0.01. Basal: basal endothelial cell medium; FBS: foetal bovine serum (10%) supplemented medium; SFM: serum-free medium. Experiments were performed using CMs from 3 donors