Fig. 6

Effects of DPSC-CMs produced in normoxia and hypoxia on monocytes. The effects of DPSC-CM Normox and DPSC-CM Hypox were tested on human CD14⁺ monocytes. Secretome analysis showing the most up-regulated soluble factors in DPSC-CM Normox and DPSC-CM Hypox related to (A) monocyte recruitment (MCP1-4, RANTES; GRO-α, I-309); (B) effects of DPSC-CM Normox and DPSC-CM Hypox on CD14⁺ monocyte migration (magnification 10×); secretome analysis showing the most up-regulated soluble factors in DPSC-CM Normox and DPSC-CM Hypox related to (C) monocyte to-macrophage differentiation (M-CSF, G-CSF, SCF) and (D) M2-like polarization (IL-4, IL-10, TGFβ1). Results are shown as mean ± SEM, t-Student test, *p ≤ 0.05; **p ≤ 0.01. FBS: foetal bovine serum (10%) supplemented medium; SFM: serum-free medium