Fig. 5

Activity of CyC-macrolides association on macrolide-resistant M. abscessus strains. Schematic checkerboard representation of association between A azithromycin or B clarithromycin and CyC 17 , and between C azithromycin or D clarithromycin and CyC 31 using WT (Control), AZM- and CLR-treated strains. Bacterial growth is represented by colored boxes, and the corresponding MIC values for macrolides and CyC are shown in gray. Based on CLSI guidelines, the green dotted lines represent the maximum macrolide concentration for which the strain is considered susceptible, and the red dotted lines correspond to the minimum concentration for which the strain is considered resistant. If bacterial growth is above the red line, the phenotype is considered resistant to macrolides, below the green line, the phenotype is considered susceptible, and between the two lines, the phenotype is intermediate. The new susceptible macrolides conditions have been indicated by a black star. S susceptible, I intermediate and R resistant