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Fig. 2 | Journal of Biomedical Science

Fig. 2

From: A novel human specific lncRNA MEK6-AS1 regulates adipogenesis and fatty acid biosynthesis by stabilizing MEK6 mRNA

Fig. 2

Identification and characterization of lncRNA MEK6-AS1. a Expression pattern of P6993 in human different tissues according to the UCSC Genome Browser. b Location of P6993 on human chromosome 17 according to the UCSC Genome Browser. c RACE assays were performed to validate the full length of MEK6-AS1. d MEK6-AS1 expression in fractionations of hAMSCs was detected by qRT-PCR. β-ACTIN mRNA served as the cytoplasmic control and U1 mRNA served as the nuclear control. e Immunofluorescence images of the distribution of MEK6-AS1 in cells was analyzed by FISH assay. 18S mRNA served as the cytoplasmic control and U6 mRNA served as the nuclear control. Scale bar: 20 µm. GAPDH was used as internal control for qRT-PCR. The quantitative data were normalized to GAPDH, n = 3. Data are shown as the mean ± SD, n = 3

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Journal of Biomedical Science

ISSN: 1423-0127