Fig. 6

YY1 was responsible for the erastin induced-upregulation of METTL3. A The expression of METTL3 mRNA was checked by qRT-PCR in HeLa and MDA-MB-231 cells after treating with erastin (2 µM) for 24 h. B The relative luciferase activity of F-Luc/R-Luc of pGL3-Basic-METTL3 promoter after treating with erastin (2 µM) for 24 h. C Venn diagram showed the overlap of transcription factors of METTL3 predicted by PROMO, JASPAR, ChIPBase and ChIP-Atlas, respectively. D The expression of YY1 mRNA was checked by qRT-PCR in HeLa and MDA-MB-231 cells after treating with erastin (2 µM) for 24 h. E The expression of YY1 protein was checked by western blot analysis in HeLa and MDA-MB-231 cells after treating with erastin (2 µM) for 24 h. F The expression of YY1 mRNA was checked by qRT-PCR in HeLa after transfecting with siRNAs of YY1 for 24 h. G The expression of YY1 protein was checked by western blot analysis in HeLa after transfecting with siRNAs of YY1 for 48 h. H The expression of YY1 protein was checked by western blot analysis (left) and quantitatively analyzed (right) in HeLa after transfecting with erastin and siRNAs of YY1 for 48 h. I Binding sites of YY1 and primer for ChIP-qPCR to the METTL3 promoter region. J ChIP-qPCR assays to examine YY1 binding to the METTL3 promoter in HeLa cells. K ChIP-qPCR assays to examine YY1 binding to the METTL3 promoter in MDA-MB-231 cells. L Schematic representation of the mutant promoter in pGL3-Basic-METTL3 reporter. M HeLa cells were co-transfected with pGL3-METTL3-WT-Luc, pGL3-METTL3-Mut-Luc, pRL-TK plasmid and si-NC or si-YY1-1 and si-YY1-2 for 48 h. Results were presented as the ratio between the activity of the reporter plasmid and pRL-TK. N Correlation between METTL3 and YY1 in cervical cancer patients (n = 306) from TCGA. O Correlation between METTL3 and YY1 in breast cancer patients (n = 1085) from TCGA. Data are presented as mean ± SD from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ns, no significant, by Student’s t test between two groups and by one-way ANOVA followed by Bonferroni test for multiple comparison