Fig. 8
From: Targeting enolase 1 reverses bortezomib resistance in multiple myeloma through YWHAZ/Parkin axis
YWHAZ is acetylated by HDAC6 and the acetylation level of YWHAZ affects mitophagy. A Identified HDAC6 peptides are shown. B Immunoblot of anti-HDAC6 immunoprecipitates from H929 cells. C Immunoblot of cell lysates or anti-YWHAZ immunoprecipitates from H929 cells with vehicle or HDAC6i treated with MG132 (10 µM) for 2 h. D Immunoblot of Flag-YWHAZ from HEK293T cells transfected with Flag‐vector, Flag‐YWHAZ, and K138R. E Representative TEM images depicted the mitochondria ultrastructure of vector-, YWHAZ-OE, YWHAZ-mutant cells. Red arrows indicate autophagic vacuoles. (Scale bars: 2 μm)