Fig. 5
HBV infection increases in EDEM3-overexpressing HepaRG cells. Supernatants of HBV-infected HepaRGEDEM3 and HepaRGC cells were harvested at 14 dpi and analyzed by ELISA (A) or subjected to immunoprecipitation with anti-preS1 antibodies, followed by viral DNA purification and quantification by real-time PCR (B). The results represent the data and SD from two independent experiments. Statistical analysis was performed by using the two-tailed student t-test (*p < 0.05, ***p < 0.001). C Total mRNA was purified from HBV-infected HepaRGEDEM3 and HepaRGC cells collected at 14 dpi and quantified by RT real-time PCR. Obtained values were normalized to GAPDH expression. The results represent the data and SD from two independent experiments. Statistical analysis was performed by using the unpaired t-test (**p < 0.01). D HepaRGEDEM3 and HepaRGC cells were transfected with pTriExHBV1.1, followed by quantification of HBV mRNA (left) and intracellular HBV nucleocapsids (right panel) by real-time PCR. The results represent the data and SD from two independent experiments. Statistical analysis was performed by using the unpaired t-test (*p < 0.05). (E) HBV-infected HepaRGEDEM3 cells were treated with 20 µM Kif for the times indicated, or maintained untreated, as control. HBV nucleocapsids were purified from cells collected at 14 dpi and DNA was quantified by real-time PCR. The results represent the data and SD from two independent experiments. Statistical analysis was measured by using the unpaired t-test (****p < 0.0001)