Fig. 2

DNA damage occurs at the affected area following SCI from 1 hpi to 3 dpi. Longitudinal spinal cord sections of SCI, sham, and naive mice were labeled for γH2AX (magenta) and DAPI (cyan). a Representative images of the affected areas of SCI, sham, and naive spinal tissue at different time points. b Timeline of cells with γH2AX foci represented as the fold change of naive mice. Cells with double-stranded DNA breaks are significantly increased in SCI groups at 1 hpi and 1 dpi compared to sham groups and at 1 hpi, 1 dpi, and 3 dpi compared to naive mice. c Timeline of cells with pan γH2AX represented as the fold change of naive mice. Cells with pan signal are significantly increased in SCI groups at 1 hpi and 1 dpi compared to sham groups and from 1 hpi up to 7 dpi compared to naive mice. n = 6–10. ANOVA with Šídák’s correction for multiple testing was applied. Error bars represent means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001