Fig. 8

PbclagX/flox parasites induced for clagX excision have perturbed NPP activity. a Giemsa images of infected RBCs harvested from mice infected with PbclagX/flox parasites that were administered either vehicle control (–Rap) or 4 mg/kg rapamycin (+ Rap), at ~ 30 hpr (n = 3 per group). b Parasitised RBCs (~ 10% parasitemia) harvested from –Rap and + Rap mice and uninfected RBCs (negative control) were resuspended in guanidium chloride (140 mM) lysis solution and the percentage of RBC lysis measured by haemoglobin absorbance, calculated relative to lysis with saponin (100% lysis) (n = 3). Statistical significance determined using a one-way ANOVA followed by Tukey’s multiple comparisons test (no significance (ns), ****p ≤ 0.0001)