Fig. 10

miR-200c inhibition and CAT treatment accelerates WH in diabetic mice. Full-thickness excisional wounds of 6 mm were performed on diabetic mice (db/db). The wounds were treated with LNA anti-miR-200c or a control sequence (anti-miR-scr) dissolved in a mixture of 30% Pluronic F-127 gel containing either LNA-scramble or anti-miR-200c (0.04 nmole/μl) in presence or absence of CAT (8UI/μl), every three days until wound closure. a The representative panels showed the rate of WH from day 0 to day 21 in the four groups of mice. b Bar graph representing the relative wound area expressed in % of wound area vs Time 0 for the indicated times and treatments. Two-way Anova, values are expressed as mean ± SEM (N = 11 anti-miR-scr w/o CAT mice, N = 10 anti-miR-scr with CAT mice, N = 11 anti-miR-200c w/o CAT mice, N = 10 anti-miR-200c with CAT mice, *P < 0.05, **P < 0.01, ***P < 0.001; asterisks of statistical significance are vs scramble ctrl at each time point, or vs the groups indicated by the lines in the bar graphs). c, d Graph and table representing the % of mice of different groups that reach a wound area < 10% compared to time zero. 100% of anti-miR-200c + CAT group reached < 10% area at 12 days after wounding, 100% of anti-miR-200c at 18 days, and 100% of CAT group at 21 days. e Graphical representation of the decline in the % of WH described by the nonlinear mixed-effects model for the different treatments from the model summary in Table 1