Fig. 5

CAT treatment decreased miR-200c-induced oxidative stress. Primary human skin FBs from HS were infected either with a lentivirus encoding miR-200c or with a control virus. Then cells were treated with 400 UI/ml equine CAT for 16 h. a Representative DHE fluorescence of FBs overexpressing miR-200c with or without CAT. Nuclei were stained with DAPI. Scale bar: 10 μm. b Scatter plot representing DHE fluorescence positivity per nuclei and expressed as fold change vs ctrl (N = 6; *p < 0.05; **p < 0.01). c Primary human skin KCs were infected either with a lentivirus encoding miR-200c or with a control virus. Then cells were treated with 400 UI/ml equine CAT for 16 h. Representative DHE fluorescence of KCs overexpressing miR-200c with or without CAT. Nuclei were stained with DAPI. Scale bar: 10 μm. d Scatter plot representing DHE fluorescence positivity per nuclei and expressed as fold change vs ctrl (N = 6; **p < 0.01; ***p < 0.001)