Fig. 6

miR-200c inhibition and CAT treatment downmodulated oxidative stress in FBs and KCs of DFU pts. a, b FBs and KCs of DFU pts were treated with 400 UI/ml for 16 h, then cells were harvested and miR-200c expression levels were quantified by RT-qPCR (N = 6 FB DB, N = 6 KC DB; *p < 0.05). c, d FBs and KCs of DFU pts were infected either with a lentivirus encoding anti-miR-200c or with a control virus (anti-miR-scr). Then cells were incubated with 400 UI/ml equine CAT for additional 16 h. Representative image of FBs and KCs diabetic pts showed that CAT treatment and anti-miR-200c individually decreased DHE signal compared to anti-miR-scr untreated cells, the co-treatment reduced oxidative stress especially in KC of DFU. e, f Oxidative stress was assayed by DHE fluorescence which was normalized for the number of DAPI-positive nuclei and expressed as fold decrease vs anti-miR-scr untreated cells. Scale bar: 10 μm (N = 6 FB DB; N = 6 KC DB; *p < 0.05; **p < 0.01; ***p < 0.001)