Fig. 7
From: miR-200c inhibition and catalase accelerate diabetic wound healing
Source data are available for this figure: Additional file1: Fig.S6
miR-200c targets are upregulated and p66shc Ser-36 phosphorylation is decreased by anti-miR-200c and CAT treatment in DFU FBs and KCs. FBs and KCs of DFU pts were infected either with a lentivirus encoding anti-miR-200c or with a control virus. Afterwards cells were incubated with 400 UI/ml CAT for additional 16 h. a, b ZEB1, SIRT1, FOXO1 and P66Shc mRNA were quantified by RT-qPCR in FBs and KCs respectively (N = 6; *p < 0.05; **p < 0.01; ***p < 0.001). c, d Representative western blots of ZEB1, SIRT1, FOXO1, P66Shc-P-Ser36 and P66Shc in FBs and KCs of DFU pts, respectively. e, f Scatter plots showing the expression levels of ZEB1, SIRT1, FOXO1, P66Shc-P-Ser36 and P66Shc proteins were evaluated by densitometric analysis normalized to vinculin protein levels in FBs and KCs (N = 6; *p < 0.05; **p < 0.01; ***p < 0.001).