Fig. 1

Diet-induced obesity affects lipid composition of AT-Exos. A Schematic diagram of AT-Exos isolation from HFD-fed or LFD-fed mice. B Representative electron microscopy images of exosomes. Scale bar, 100 nm. C The particle size of the AT-Exos measured by Nanoparticle tracking analysis. D The exosome-related protein markers CD63, HSP70 and CD9 measured by western blot in AT-Exos. These blots are representative of three independent replicate experiments. E Quantification of AT-Exos released from adipose tissue (per gram per hour) from HFD-fed and LFD-fed mice, as determined by Nanoparticle Tracking Analysis. n = 6 per group. F-I Partial Least-Squares Discriminant Analysis (PLS-DA) of lipid metabolites in AT-Exos from different origins, including WAT-Exos from HFD-fed and LFD-fed mice (panel f), BAT-Exos from HFD- and LFD-fed mice (panel g), BAT-Exos and WAT-Exos from LFD-fed mice (panel h), BAT-Exos and WAT-Exos from HFD-fed mice (panel i). J The heatmap shows relative content of different lipid types in WAT-Exos and BAT-Exos of wild-type B6 mice fed LFD or HFD for 16 weeks. n = 6 per group. All data are presented as mean ± SEM. *P < 0.05 and**P < 0.01