Fig. 2

Diet-induced obesity affects miRNAs composition of AT-Exos. A Venn diagram between miRNA expression in vWAT-Exos using a fold change cutoff of 2 or greater (FC > 2), a value cutoff of P = 0.0001 and a norm value cutoff of 100. B-D qPCR validation of microarray data for miR-200a-3p, miR-200b-3p, miR-125a-5p, miR-455-3p and miR-125b-5p expression in vWAT-Exos (panel b), iWAT-Exos (panel c) and BAT-Exos (panel d) from HFD-fed mice relative to LFD-fed mice. Data are displayed as the expression of each miRNA in AT-Exos from HFD-fed mice relative to that assayed in LFD-fed mice, n = 6 per group. E–F qPCR validation of microarray data for miR-200a-3p, miR-200b-3p, miR-125a-5p, miR-455-3p and miR-125b-5p expression in vWAT-Exos relative to iWAT-Exos (panel e) or BAT-Exos (panel f) from HFD-fed mice. Data are displayed as the expression of each miRNA in vWAT-Exos from HFD-fed mice relative to that assayed in iWAT-Exos or BAT-Exos, n = 6 per group. G Validated mRNA target for each miRNA extracted from the miRTarBase are displayed as well as their respective expression level in 3T3L1 cells after stimulated by vWAT-Exos from HFD-fed mice compared with LFD-fed mice. H Venn diagram of the differential abundance of the identified miRNAs in vWAT-Exos from HFD-fed mice compared to LFD-fed mice using a norm value cutoff of 10. The number of “exclusive set” (red or blue area) and “common set” (gery area) of miRNAs is indicated on the diagram. I Based on the prediction of target genes for miRNA differences between vWAT-Exos from HFD-fed and those from LFD-fed mice, significantly enriched GO-terms/KEGG pathways were defined. The heatmap represents the log10 value of target genes number per GO term or per KEGG pathways. The red boxes highlight the significant GO-terms/KEGG pathways of interest. All data are presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001