Fig. 4

Single-cell transcriptome analysis of tumor-infiltrating immune cells in TMZ-sensitive and TMZ-resistant GBM. A UMAP plots of lymphoid and myeloid cells from tumor-infiltrating leukocytes and microglia. A total of 26,039 tumor-infiltrating CD45⁺ cells were harvested on post–tumor inoculation day 28, and scRNA-seq analysis was performed. A total of 6,150 cells from four lymphoid clusters were divided into nine lymphoid clusters (C1–C9). A total of 19,889 tumor-infiltrating CD45⁺ cells from 10 myeloid clusters were divided into 12 clusters (10 myeloid clusters and two B cell clusters). B Bubble plot map of expression patterns of selected marker genes across indicated lymphoid clusters and C myeloid clusters. Color scale depicts expression level. D UMAP plots of lymphoid and E myeloid cells from GL261 and GL261/TMZ-R GBM-bearing mice treated with isotype control antibody or IL-19 antibody. F Proportions of B cells (BCs), TCs, NK cells, microglia (MG), monocytes/macrophages (Mo/Mϕ), DCs, MCs in GL261 and GL261/TMZ-R treated with isotype control or IL-19 antibody