Fig. 2

Resocialization is insufficient to rescue receptive social behavioral changes by adolescent SI. A Schematic diagram illustrating the experimental pipeline. Male mice were housed either in groups or alone between P28 and P34. SI mice were regrouped (rGH) until P55 and GH mice were always kept in a group. A series of behavioral tests consisting of the free interaction test, object exploration, three-chamber test, and habituation test were performed between P56 and P59. B Top, schematic representation of the free interaction test. Male rGH mice spend more time interacting with the novel mice compared with male GH mice [mouse number: GH: n = 10; SI: n = 11; two-tailed unpaired Student’s t-test; t₍₁₉₎ = 2.91, P = 0.0089, 95% CI (7.70–46.98)]. C Top, schematic representation of the object exploration test. Bottom, the time spent exploring the novel object was comparable between male rGH and GH mice [mouse number: GH: n = 10; SI: n = 11; two-tailed unpaired Student’s t-test; t₍₁₉₎ = 0.38, P = 0.71, 95% CI (−10.69 to 15.47)]. D Top, schematic representation of the habituation test. Bottom, male rGH and GH mice exhibited similar behavioral habituation to repeated social stimulation [mouse number: GH: n = 10; SI: n = 11; two-way RM ANOVA, trial: F_(3,57) = 16.49, P < 0.0001; housing condition: F_(1,19) = 0.38, P = 0.5472; trial × housing condition interaction: F_(3,57) = 0.52, P = 0.6686]. E Left, schematic representation of the 3-chamber sociability test. Middle, male rGH and GH subject mice spent significantly more time interacting with the wire cage containing the juvenile stimulus mouse (S) than the empty wire cage (E). Right, the discrimination index (stimulus minus empty) was comparable between male rGH and GH subject mice in the sociability test [mouse number: GH: n = 10; SI: n = 11; two-tailed unpaired Student’s t-test; t₍₁₉₎ = 0.63, P = 0.536, 95% CI (−0.06 to 0.11)]. F Left, schematic representation of the 3-chamber social novelty preference test. Middle, male rGH and GH subject mice spent significantly more time sniffing the cage containing the novel mouse (N1) than the familiar mouse (F). Right, the discrimination index (novel 1 minus familiar) was comparable between male rGH and GH subject mice in the social novelty preference test [mouse number: GH: n = 10; SI: n = 11; two-tailed unpaired Student’s t-test; t₍₁₉₎ = 1.37, P = 0.188, 95% CI (−0.20 to 0.04)]. G Left, schematic representation of the 3-chamber SRM test. Middle, male GH, but not male dGH, subject mice spent significantly more time sniffing the cage containing the novel mouse (N2) than the familiar mouse (N1). Right, the discrimination index (novel 2 minus familiar) of male rGH subject mice was significantly less than male GH subject mice in the SRM test [mouse number: GH: n = 10; SI: n = 11; two-tailed unpaired Student’s t-test; t₍₁₉₎ = 3.09, P = 0.006, 95% CI (−0.48 to −0.09)]. Data are presented as mean ± SEM. **P < 0.01 and ***P < 0.001. Panels B–G were created with BioRender.com