Fig. 2

I/V relationships in HERG WT and mutants. The I/V graphs are derived by voltage clamp recordings showing WT, A561V, G628S and L779P activation currents recorded in basal condition (black square) and after 10 µM ICA-105574 perfusion (red dot). HERG activation currents (examples in Figure S5) were recorded in whole-cell configuration and measured at the end of the second pulse (steady-state currents), normalised to the maximum evoked current and plotted as a function of voltage to construct the steady-state activation I/V plot. Each value is represented as mean ± s.e.m. Statistical analyses were conducted using two-way ANOVA with Šidák post test. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001. WT: n = 5, A561V: n = 6, G628S: n = 6, L779P: n = 4